WebAug 31, 2016 · Four different stationary phases were tested (BEH, BEH 2-EP, CSH PFP and HSS C18). The results are shown in Fig. 2. CSH PFP stationary phase was not suitable for the separation under selected conditions due to coelution of two critical pairs β-; γ-T and β-; γ-T 3 and very low retention (analysis time < 0.8 min) which confirmed our expectations. WebDec 1, 2014 · Retention times using methanol are appreciably longer due to its lower solvent strength compared to acetonitrile. The use of acetonitrile or methanol in conjunction with additional “orthogonal” columns (CSH phenyl or CSH PFP) can help increase selectivity differences for difficult separations of critical pairs [49].

CSH File: How to open CSH file (and what it is)

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