WebJan 1, 2011 · Labeling nuclear DNA using DAPI. A number of fluorescent stains are available that label DNA and allow easy visualization of the nucleus in interphase cells … WebApr 10, 2024 · The Ug99-effective stem rust resistance gene Sr48 was mapped to chromosome 2A based on its repulsion linkage with Yr1 in an Arina/Forno recombinant inbred line (RIL) population. Attempts to identify markers closely linked to Sr48 using available genomic resources were futile. This study used an Arina/Cezanne F5:7 RIL …
Fluorescent Staining of Living Plant Cells SpringerLink
WebOct 10, 2015 · SR2200 was excited with a 405-nm laser line and emission recorded between 415 and 476 nm (405/415–476); similar settings were used to detect DAPI. For fluorescent proteins, the following excitation/emission wavelengths were used: CFP (458/473–552), GFP (488/505–540), YFP (514/517–597), dsRed variants (561/565–615). WebDAPI 359 461 325,360,405,407 Hoechst 33258 352 461 Hoechst 33342 350 461 SYTOX Blue 431 480 YOYO-1 491 509 SYTOX Green 504 523 TOTO-1, TO-PRO-1 509 ... moves back down to the lower energy level (Gs) (3). In the case of a tandem fluorescent dye, after excitation of the electron by a laser (1 - 2), energy is released by an electron in the donor ... fishing terms and phrases
Alternatives to DAPI staining: imaging and counting …
DAPI's blue emission is convenient for microscopists who wish to use multiple fluorescent stains in a single sample. There is some fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP) but the effect of this is small. See more DAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine-rich regions in DNA. It is used extensively in fluorescence microscopy. … See more This DNA fluorescent probe has been effectively modeled using the time-dependent density functional theory, coupled with the IEF version of the polarizable continuum model See more The Hoechst stains are similar to DAPI in that they are also blue-fluorescent DNA stains which are compatible with both live- and fixed-cell applications, as well as visible using the … See more DAPI was first synthesised in 1971 in the laboratory of Otto Dann as part of a search for drugs to treat trypanosomiasis. Although it was … See more When bound to double-stranded DNA, DAPI has an absorption maximum at a wavelength of 358 nm (ultraviolet) and its emission maximum is at 461 nm (blue). Therefore, for fluorescence microscopy, DAPI is excited with ultraviolet light and is detected through a … See more DAPI can be used for fixed cell staining. The concentration of DAPI needed for live cell staining is generally very high; it is rarely used for live … See more • DNA binding ligand • Hoechst stain • Lexitropsin • Netropsin • Pentamidine See more WebJul 6, 2024 · 7-AAD is a fluorescent probe and intercalating agent. Like DAPI, it has a strong affinity for binding double stranded DNA. Unlike DAPI which binds to A-T rich regions, 7-AAD selectively binds to G-C rich … WebDescription: Fluorescent DNA dye; DAPI can be used for cell staining and assessing apoptosis; suitable for flow cytometry; DAPI mw and DAPI ex/em, provided. Alternative … fishing terms glossary