Phospho eif4b

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August undefined, 2024

WebAll lanes : Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823) at 1/50000 dilution Lane 1 : 293T cell lysate, untreated Lane 2 : 293T cell lysate, treated with AP Lysates/proteins at 10 µg per lane. Secondary All lanes : goat anti-rabbit HRP at 1/2000 dilution Predicted band size: 69 kDa Observed band size: 69 kDa WebApr 6, 2024 · The protein expression of mTOR, phospho-mTOR, p70 S6 kinase (p70 S6K), phospho-p70 S6K, eIF4B, phospho-eIF4B, eIF4G, 4E-BP1, phospho-4E-BP1, eIF4E, phospho-eIF4E, and Rpb1 in the liver was assessed using western blotting. Actin was used as the loading control. (Specific antibody information is provided in the Supplementary Materials.)

Anti-eIF4B (phospho S422) antibody (ab59300) Abcam

Web人载脂蛋白A4(Apo-A4)ELISA试剂盒；英文名称： Human Apo-A4 ELISA Kit ；实验名称：人载脂蛋白A4(Apo-A4)ELISA试剂盒；实验别名：人载脂蛋白A4(Apo-A4)ELISA试剂盒 48T/96T ；规格： 48T/96T 报价人载脂蛋白A4(Apo-A4)ELISA试剂盒实验目的：本试剂盒仅供研究使用，用于用于测定血清、血浆及相关液体样本中待测物质 ... WebEIF4B Antibody (phospho-Ser422) LS-C826726 Antibody: EIF4B Rabbit anti-Human Polyclonal (pSer422) Antibody . Reactivity: Mouse, Rat, Human . Applications: ELISA, IF, IHC, WB . Format: Unconjugated . Price: 1 mg/$357. Select. EIF4B Antibody LS-C334046 Antibody: EIF4B Rabbit anti-Human Polyclonal Antibody ... philip columbus

Phospho-EIF4B (Ser406) antibody (28779-1-AP) Proteintech

WebPhospho-EIF4B (Ser406) EIF4B (Eukaryotic Translation Initiation Factor 4B) is a Protein Coding gene. Among its related pathways are Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S and Viral mRNA Translation. Gene Ontology (GO) annotations related to this gene include nucleic acid binding ... WebAnti-eIF4B (phospho S422) antibody See all eIF4B primary antibodies Description Rabbit polyclonal to eIF4B (phospho S422) Host species Rabbit Tested applications Suitable for: IHC-P, WB, ICC/IF more details Species reactivity Reacts with: Mouse, Human Immunogen Synthetic peptide corresponding to Human eIF4B aa 350-450 (phospho S422). T-G-SP-E-S WebAll lanes : Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823) at 1/50000 dilution Lane 1 : 293T cell lysate, untreated Lane 2 : 293T cell lysate, treated with AP … philip comeyne

Phospho-eIF4B (Ser406) Polyclonal Antibody (28779-1-AP)

Phosphorylation of eukaryotic initiation factor-2α (eIF2α ... - Nature

WebJun 8, 2006 · eIF4B Ser422 phosphorylation persists in cells lacking S6K1 and S6K2 To further corroborate the existence of an eIF4B kinase that is distinct from S6K in cells … WebThe eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. philip comerfordWebEIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins … philip combs

"WebWestern blot analysis of extracts from HeLa cells, untransfected or transfected with SignalSilence ® eIF4B siRNA #6416, using Phospho-eIF4B (Ser422) Antibody (upper) or eIF4B Antibody #3592 (lower). 48 hours following transfection, cells were treated with Rapamycin (50 nM) and U0126 (10 uM) as indicated. " - Phospho eif4b

Phospho eif4b

WebPhospho-EIF4B-S422 Rabbit pAb (AP0278) Datasheet SDS Tested applications: WB IHC Reactivity: Human, Mouse Western blot analysis of extracts from HeLa cells using phospho-EIF4B-S422 antibody (AP0278). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. WebAnti-eIF4B (phospho S422) antibody See all eIF4B primary antibodies Description Rabbit polyclonal to eIF4B (phospho S422) Host species Rabbit Tested applications Suitable for: …

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WebRecombinant Anti-eIF4B (phospho S406) antibody [EPR7683] - BSA and Azide free (ab248698) Datasheet Certificate of Compliance Submit a review Submit a question Product price, shipping and contact information Currently … WebJan 5, 2024 · The translation initiation factor eIF4A is a member of the “DEAD box” family of helicases that catalyze the ATP-dependent unwinding of RNA duplexes and facilitates 43S ribosome complex scanning through highly structured regions of the 5′-untranslated region (UTR) and recognition of the AUG initiation codon.

WebOct 6, 2008 · Insulin stimulation resulted in phosphorylation of eIF4B as detected by both the phospho-eIF4B Ser422 antibody as well as the phospho-PKB substrate antibody (Figure 5a, lanes 1 and 2). WebNov 18, 2016 · The activity of eIF4B is modulated by phosphorylation, which is mediated by two major signaling pathways, the MEK1/2-ERK1/2-RSK pathway and the PI3K-Akt-mTORC1-S6K pathway [8], [9], [11], [12]. Overexpression or depletion of eIF4B affects cell survival and proliferation [13], [14].

WebAug 4, 2024 · Phosphorylated eIF4B levels are normalized against total eIF4B, then for loading (Tubulin) and shown as fold change over control (untreated neurons). Data are … WebPhospho-EIF4B-S422 Rabbit pAb (AP0278) Datasheet SDS Tested applications: WB IHC Reactivity: Human, Mouse Western blot analysis of extracts from HeLa cells using …

WebPhospho-eIF4E (Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at serine 209. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser209 of human eIF4E.

Web酶联生物为您提供大鼠热休克蛋白β6(hspβ6)检测试剂盒的参数及2024年最新报价，厂家专业的大鼠热休克蛋白β6(hspβ6)检测试剂盒售后服务团队，是您值得信赖的合作伙伴 philip comfortWebPhospho-EIF4B-S422 Rabbit pAb (AP0775) Datasheet SDS Tested applications: WB Reactivity: Human Western blot analysis of extracts of HeLa cells, using Phospho-EIF4B-S422 antibody (AP0775) at 1:2000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. philip comfort bible philip comerford architectWebJan 9, 2012 · The lower lane shows tubular cell expression of actin under similar conditions. A bar diagram shows cumulative data of four sets of experiments in the right panel. B. Representative gels (in duplicate) showing tubular cell expression of phospho-eIF4B under control (vector) and HIV infection states (upper lane). philip commercial guildfordWebAug 17, 2014 · Phospho-proteomics identified the mTOR pathway to be of interest in EGFR inhibitor resistance. In our studies, combining gefitinib and temsirolimus decreased cell growth and survival in a synergistic manner. Our data identified eIF4B, as a potentially key fragile point in EGFR and mTOR inhibitor synergy. philip comoWebHuman Phospho-eIF4B (S422) Antibody Summary Species Reactivity Human Specificity Detects human eIF4B when phosphorylated at S422 Source Monoclonal Rat IgG 2A Clone # 866049 Purification Protein A or G purified from hybridoma culture supernatant Immunogen Phosphopeptide containing the human eIF4B S422 site Formulation philip comynWebeIF4B (eukaryotic translation initiation factor 4B), an RNA binding protein, is essential for the binding of mRNA to the 43S pre-initiation complex, which consists of the 40S ribosomal … philip comper