Two-photon intravital imaging
WebTreatment with dexmedetomidine (DEX), an already FDA-approved alpha-2 adrenergic receptor agonist, is demonstrated to reduce tissue oscillation frequency and amplitude in … WebJan 16, 2024 · Biomimetic fluorescent nanoprobes capable of emitting near-infrared (NIR) fluorescence (λmax ≈ 720 nm) upon excitation of 800 nm light were developed. The key …
Two-photon intravital imaging
Did you know?
WebTwo-Photon Imaging. Imaging can begin as early as 1 d after injection of the CNS-1 cells. 23. Anesthetize the mouse with 200 mg/kg Avertin intraperitoneally. Place the … WebMaterials and Methods U-DiFC Instrument. A schematic and photograph of the U-DiFC instrument are shown in Figure 1B and C.The optical design is based on a standard epi–illumination and fluorescence detection geometry ().We used a 980 nm laser (DL980-200, CrystaLaser LLC, Reno, NV) to illuminate a spot on the sample (2 mm in diameter) …
WebUsing intravital imaging, we have captured, for the first time in a live mammal, ... Inhibition of proliferation was monitored by time-lapse imaging via two-photon laser scanning microscopy described below. K14-rtTA;tetO-Cre;Rosa-stop-tdTomato;K14-H2BGFP mice were used as controls. WebLead the development and implementation of new technologies and protocols for advanced in two-photon imaging applications (e.g. functional imaging, labeling methods) Develop …
WebApr 27, 2024 · The new technologies, including two-photon microscopy (2PM), have considerably improved the in vivo study of pathophysiological processes, allowing the … WebWorking on regulation of the mammary gland by resident immune cells and stem cell behaviour during mammary gland morphogenesis. Techniques include 3D confocal and 2 …
WebProcess, analyze, and quantify videos of living animals from two-photon Intravital imaging. 2D/3D Image Processing and Analysis of Histological Immunohistochemistry whole-slide scans (human, mice). (Enhancement, Registration, Segmentation, Reconstruction).
Web图2 多光子活体成像结果 a 台式三光子显微镜对小鼠皮层神经元的成像结果；b台式双光子显微镜（左）和微型化双光子显微镜（右）对小鼠皮层神经元的成像结果[2] 近年来，各种 … generic grocery 80sWebDec 30, 2024 · With regard to traditional intravital techniques, such as one-photon confocal or two-photon microscope, the limited imaging depth (<360 µm for conventional one-photon confocal imaging in the visible or NIR-I window) [32,46,47] and long acquisition time (1.25 to 5 s per frame) restrain the in vivo application. generic grocery bannerWebJun 24, 2024 · Intravital 2-photon imaging reveals distinct morphology and infiltrative properties of glioblastoma-associated macrophages. ... Open-skull window 2-photon … death explainedWebI have been interested in structure and dynamics of DNA and proteins and used photon- and fluorescence correlation spectroscopy and single molecule fluorescence spectroscopy for this aim. Major projects are on Gold Nanoparticles for drug delivery and cancer therapy; Interactions between dendritic and natural killer cells in vivo by multiphoton intravital … death experiences videoWebPAI can also effectively activate M2 receptors using two-photon excitation with near-infrared light, which overcomes the scattering and low penetration of short-wavelength illumination, and offers new opportunities for intravital imaging and control of cardiac function. death.exe virusWebTwo-photon intravital imaging is a powerful method by which researchers are able to directly observe biological phenomena in live organisms. Researchers in various … generic grocery couponsWebApr 11, 2024 · Fluorescence Lifetime Imaging for Diagnostic and Therapeutic Intravital Microscopy; Fluorescence Lifetime Imaging Microscopy for Quantitative Biological Imaging; Polarimetric multiphoton fluorescence microscopy in molecular and biological media; Synchrotron Based Fourier Transform Infrared Microspectroscopy and Fluorescence … death extortion scam